Research article summary (published 29 Sep 2009):

Determination of intrinsic monod kinetic parameters for two heterotrophic tetrachloroethene (PCE)-respiring strains and insight into their application.

Full Abstract

A complete set of mathematically identifiable and meaningful kinetic parameters estimates is needed to accurately describe the activity of individual populations that dehalorespire tetrachloroethene (PCE) and other chlorinated ethenes. These data may be difficult to extract from the literature because kinetic parameter estimates obtained using mixed cultures may reflect the activity of multiple dehalorespiring populations, while those obtained at low initial substrate-to-biomass ratios (S(0)/X(0)) are influenced by culture history and are generally not relevant to other systems. This study focused on estimation of electron donor and acceptor utilization kinetic parameters for the heterotrophic dehalorespirers Desulfuromonas michiganensis strain BB1 and Desulfitobacterium sp. strain PCE1. Electron acceptor utilization kinetic parameters that are identifiable and independent of culture history, i.e., intrinsic, could be estimated at S(0)/X(0) >or= 10, with both concentrations expressed as chemical oxygen demand (COD). However, the parameter estimates did not accurately describe dechlorination kinetics at lower S(0)/X(0) ratios. The maximum specific substrate utilization rates (q(max)) and half-saturation constants (K(S)) for PCE and trichloroethene (TCE) estimated for the two heterotrophic strains are higher than the values reported for Dehalococcoides cultures. These results suggest that the natural niche of Dehalococcoides strains that can metabolize a range of chlorinated ethenes may be to respire dichloroethene and vinyl chloride produced by Desulfuromonas and Desulfitobacterium strains or other populations that dechlorinate PCE and TCE at faster rates. Few data exist on the electron donor utilization kinetics of heterotrophic dehalorespirers. The results of this study suggest that Desulfuromonas and Desulfitobacterium strains should be able to compete for organic electron donors with other heterotrophic populations in the subsurface. (c) 2009 Wiley Periodicals, Inc.

Author information

Author/s: Huang, Deyang (D); Becker, Jennifer G (JG);

Affiliation: Department of Civil and Environmental Engineering, University of Maryland, College Park, Maryland, USA.

Journal and publication information

Publication Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.

Journal: Biotechnology and bioengineering (Biotechnol Bioeng), published in United States. (Language: eng)

Reference: 2009-Oct; vol 104 (issue 2) : pp 301-11

Dates: Created 2009/08/27; Completed 2009/10/15;

PMID: 19593756, status: MEDLINE (last retrieval date: 10/15/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Desulfitobacterium - metabolism Desulfuromonas - metabolism Kinetics Models, Theoretical

Models, Theoretical Oxidation-Reduction Tetrachloroethylene - metabolism Vinyl Chloride - metabolism

Associated Chemicals: Tetrachloroethylene (127-18-4) ; Vinyl Chloride (75-01-4)

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