Research article summary (published 30 Oct 2009):

Mineralized tissue formation by BMP2-transfected pulp stem cells.

Full Abstract

Previously, in vitro differentiation of odontoblasts was shown for dental pulp stem cells (DPSCs) transfected with bone morphogenetic protein-2 (Bmp2). For this study, we hypothesized that such cells also show potential for mineralized tissue formation in vivo. DPSCs were transfected with Bmp2 and seeded onto a ceramic scaffold. These complexes were cultured in medium without dexamethasone, and thereafter placed subcutaneously in nude mice for 1, 4, and 12 weeks. Samples were evaluated by histology and real-time PCR for osteocalcin, bone sialoprotein, dentin sialophosphoprotein, and dentin matrix protein 1. Results indicated that only the transfected DPSCs showed obvious mineralized tissue generation, and 12 weeks of implantation gave the highest percentage of mineralized tissue formation (33 +/- 7.3% of implant pore area). Real-time PCR confirmed these results. In conclusion, Bmp2-transfected DPSCs effectively show mineralized tissue formation upon ectopic implantation.

Author information

Author/s: Yang, X (X); van der Kraan, P M (PM); Bian, Z (Z); Fan, M (M); Walboomers, X F (XF); Jansen, J A (JA);

Affiliation: Key Lab for Oral Biomedical Engineering of Ministry of Education and Department of Endodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei Province, P.R. China.

Journal and publication information

Publication Type: Journal Article

Journal: Journal of dental research (J Dent Res), published in United States. (Language: eng)

Reference: 2009-Nov; vol 88 (issue 11) : pp 1020-5

Dates: Created 2009/10/15; Completed 2009/10/29;

PMID: 19828890, status: MEDLINE (last retrieval date: 10/29/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adenoviridae - genetics Animals Bone Morphogenetic Protein 2 - genetics; physiology Calcification, Physiologic - genetics; physiology Cells, Cultured Ceramics - chemistry Dental Pulp - cytology Extracellular Matrix Proteins - analysis Genetic Vectors - genetics Hydroxyapatites - chemistry Mice

Mice, Inbred BALB C Mice, Nude Osteocalcin - analysis Phosphoproteins - analysis Protein Precursors - analysis Sialoglycoproteins - analysis Stem Cells - physiology Subcutaneous Tissue - pathology; surgery Time Factors Tissue Scaffolds Transfection

Associated Chemicals: Bmp2 protein, mouse (0) ; Bone Morphogenetic Protein 2 (0) ; Ceramics (0) ; Dmp1 protein, mouse (0) ; Dspp protein, mouse (0) ; Extracellular Matrix Proteins (0) ; Hydroxyapatites (0) ; Phosphoproteins (0) ; Protein Precursors (0) ; Sialoglycoproteins (0) ; bone sialoprotein (0) ; hydroxyapatite-beta tricalcium phosphate (0) ; Osteocalcin (104982-03-8)

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